Blog

Description

Blog

 

Check out our blog for research news, application tips, and information in the field of genetic research.

 

PCR-based Ribotyping of Clostridium difficile

15 OCT 2018

Clostridium difficile (C. difficile) is a commonly identified healthcare-associated infection that is sometimes difficult to treat and can in some cases be fatal. The ability to identify and track particular strains during outbreaks is important to understanding the epidemiology. There have been many methods used for typing C. difficile such as pulsed-field gel electrophoresis and restriction endonuclease analysis, but PCR ribotyping has emerged as a simple and cost-effective method.

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Meet Us at ASHG 2018

24 SEP 2018

We will be exhibiting at this year's American Society of Human Genetics from October 17-19, 2018 in San Diego, California and look forward to meeting those attending at Booth 811. On Wednesday, October 17, 2018 from 12:30 PM - 1:45 PM in the San Diego Convention Center Room 28D, Upper Level we will have an education event — Introducing NEXTGENPCR presented by NEXTGENPCR inventor, Gert de Vos.

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Novallele™ Assay Amplification Reaction Using NEXTGENPCR

17 SEP 2018

NEXTGENPCR has been shown to speed the PCR amplification of DNA. In this technical note, we use NEXTGENPCR in the amplification step of our Novallele assay for the G6PC gene. Using the advanced mode function of NEXTGENPCR, we were able to reduce the time for a 35 cycle amplification to only 4 minutes and 30 seconds.

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Enzymes for PCR — Abundant Choices

10 SEP 2018

When thinking about the enzyme used in standard end-point PCR, typically Taq DNA polymerase, isolated from Thermus aquaticus, comes to mind. However, there are many options. There are enzymes isolated from other thermostable organisms such as Thermococcus zilligii, Pyrococcus furiosus, and Thermus filiformis.

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Six Tips to Combat Post-holiday Blues in the Lab

27 AUG 2018

We've all been there. Coming off of that sand-in-your-toes high and jumping into a daunting amount of work you said you’d do when you got back from vacation. Summer is nearly over, and it’s time to roll up our sleeves and start attacking those to do lists to achieve your goals by the end of the year. Here are a few motivating tips to help you get back on track:

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End-Point PCR Amplification Methods

20 AUG 2018

Polymerase chain reaction, or PCR, that was developed in 1983 by Kary Mullis, has become an essential tool for molecular biology. It allows logarithmical amplification of deoxyribonucleic acid (DNA) from a very small amount of starting material. PCR amplification is used for a variety of applications, including amplifying small fragments for cloning, genotyping, whole genome amplification, Sanger sequencing, and next-generation sequencing. 

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An Overview of Next Generation Sequencing

13 AUG 2018

Next Generation Sequencing (NGS) is also known as high-throughput sequencing. It allows for rapid sequencing of whole genomes or specific PCR amplicons of interest. NGS can be used for sequencing DNA or RNA from various sources.

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STRs — Ideal for Human Identification

06 AUG 2018

Microsatellites, also known as Short Tandem Repeats (STRs), are stretches of DNA that are made up of two to six nucleotides-long repeats adjacent to each other. The stretches of repeats are variable in length, from a few to hundreds of repeats.

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Top Ten Most Asked Questions About NEXTGENPCR

30 JUL 2018

The ability of the novel NEXTGENPCR thermocycler to perform ultrafast cycling brings questions from scientists. Here are the top ten questions and answers.

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NEXTGENPCR Amplifies the Entire BRCA1 Gene in 10 Minutes [POSTER]

16 JUL 2018

With optimization and the use of a fast enzyme, the NEXTGENPCR thermocycler amplified a 100 bp fragment in less than 2 minutes. But what about larger fragments? A poster presented at the European Society of Human Genetics 2016 Annual Meeting demonstrated how the NEXTGENPCR thermocycler amplified the entire BRCA1 gene within 10 minutes using 29 primer pairs. The PCR was followed by Sanger sequencing.

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PCR in 1 Minute and 59 Seconds – How It Was Done

09 JUL 2018

DNA amplification using PCR is a necessary step in most molecular biology procedures. Molecular Biology Systems (MBS) developed NEXTGENPCR, a novel thermocycler that uses distinct thermal zones to denature, anneal and extend, eliminating ramp times. The thermal zones are maintained at a set temperature, and the samples move between thermal zones so that only the sample temperature changes.

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Ten Tips for Preventing Amplicon Contamination

02 JUL 2018

Amplicon contamination poses a real problem for laboratories performing PCR-based experiments. Amplicon contaminants can come from any RNA or DNA, from a previous PCR amplification, or from a laboratory procedure, such as a plasmid preparation or in vitro transcription.

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Writing A Quality Scientific Abstract

11 JUN 2018

As a scientist preparing for a journal article, poster presentation, or talk, you are likely required to write an abstract about the content. Make sure to check the guidelines and limitations specific to the journal, conference, or venue regarding the word limit and format, but some basic advice holds true for writing any scientific abstract.

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NEXTGENPCR - Discover How It Works

04 JUN 2018

Historically, PCR instrumentation has relied on Peltier technology to power the process of heating and cooling samples, resulting in similar run times between instruments and few significant improvements in run times over the last 30 years. The NEXTGENPCR thermocycler introduces a new technology that enables samples to heat and cool almost instantly.

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Meet the Inventor of NEXTGENPCR [Interview Part 2 of 2]

30 APR 2018

In last week's blog, we began a very interesting interview with Gert de Vos, the inventor of NEXTGENPCR and Director at Molecular Biology Systems, B.V. (MBS). This week Dana Pfister Sullivan, Product Manager at Canon BioMedical, finishes our interview with Gert about the ultrafast NEXTGENPCR.

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Meet the Inventor of NEXTGENPCR [Interview Part 1 of 2]

23 APR 2018

Recently, Dana Pfister Sullivan, a product manager at Canon BioMedical, sat down with Gert de Vos, the inventor of the NEXTGENPCR instrument and Director at Molecular Biology Systems, B.V. (MBS). Gert has master’s degrees in biology and physics from Leiden University in the Netherlands.

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Resources

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