AssayDetailPortlet

CYP2C19 c.819+2T>A Novallele Genotyping Assay

Gene symbol

CYP2C19 

Nucleotide change

c.819+2T>A 

Protein change

NCBI 1000 Genomes Browser

rs72558186

Assay catalog number

40081 

Control set catalog number(s)

40445

The CYP2C19 c.819+2T>A Novallele Genotyping Assay identifies a single nucleotide change within the CYP2C19 gene associated with a common allele, CYP2C19*7. The CYP2C19*7 allele causes splice donor mutation, which results in poor drug metabolism. This impact on drug metabolism is characterized by deficient synthesis of cholesterol, steroids, and other lipids.

Variant information

The CYP2C19 c.819+2T>A Novallele Genotyping Assay is used to amplify and identify specific genetic polymorphisms or mutations. This assay provides scientists with a simple way to quickly genotype this locus from isolated human genomic DNA. The assay contains functionally-verified oligonucleotide primers and unlabeled probes necessary for PCR and subsequent allele identification by DNA melting. For Research Use Only. Not for use in diagnostic procedures.

Catalog number

 

40081 

 

Gene symbol

 

CYP2C19 

Alias

 

Cytochrome P450, Family 2, Subfamily C, Polypeptide 19,CYP2C, Cytochrome P450, Subfamily IIC (Mephenytoin 4-Hydroxylase), Polypeptide 19, Cytochrome P450-11A, Cytochrome P450-254C, Mephenytoin 4-Hydroxylase, (R)-Limonene 6-Monooxygenase, (S)-Limonene 6-Monooxygenase, (S)-Limonene 7-Monooxygenase, CYPIIC17, CYPIIC19, CPCJ, P450C2C, P450IIC19, Cytochrome P-450 II C, Cytochrome P450, Flavoprotein-Linked Monooxygenase, Mephenytoin 4'-Hydroxylase, Microsomal Monooxygenase, S-Mephenytoin 4-Hydroxylase, Xenobiotic Monooxygenase, EC 1.14.13, EC 1.14.13.48, EC 1.14.13.49, EC 1.14.13.80, EC 1.14.14.1 

Nucleotide change

 

c.819+2T>A 

Protein change

 

Allele information

 

 

Application area

 

Pharmacogenetics

Cardiovascular

Variant type

 

SNP

Cytogenetic location

 

10q23.3

SNP type

 

Missense 

 

NCBI 1000 Genomes Browser

 

rs72558186

dbSNP ID

 

ClinVar

 

Online Mendelian Inheritance in Man (OMIM)

 

 

Drug interaction

 

amitriptyline, clopidogrel, doxepin, imipramine, Selective serotonin reuptake inhibitors, trimipramine, diazepam, sertraline, voriconazole, citalopram, escitalopram, clomipramine, esomeprazole, lansoprazole, omeprazole, rabeprazole 

Product specifications

The CYP2C19 c.819+2T>A Novallele Genotyping Assay is compatible with any thermocycler capable of high-resolution melting (HRM). Each assay comprises a single tube containing the primers and probes optimized to detect the target. Each tube contains 600 µl, which is enough for 166 reactions. The assay is stable for 6 months at 2–8ºC.

Genotyping analysis

The CYP2C19 c.819+2T>A Novallele Genotyping Assay identifies a SNP of c.819+2T>A. The graphs display the homozygous wild type in black, the heterozygous variant in blue, and the homozygous variant in red. The data were generated using the companion Novallele Control Set.

Resources

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