AssayDetailPortlet

CYP3A5 c.1035dupT Novallele Genotyping Assay

Gene symbol

CYP3A5 

Nucleotide change

c.1035dupT 

Protein change

NCBI 1000 Genomes Browser

rs41303343

Assay catalog number

40317 

Control set catalog number(s)

40671

The CYP3A5 c.1035dupT Novallele Genotyping Assay identifies a duplication within the CYP3A5 gene. This family of P450 proteins metabolizes drugs as well as steroid hormones. Research demonstrates mutations within this gene are related to tacrolimus dose selection and engraftment syndrome.

Variant information

The CYP3A5 c.1035dupT Novallele Genotyping Assay is used to amplify and identify specific genetic polymorphisms or mutations. This assay provides scientists with a simple way to quickly genotype this locus from isolated human genomic DNA. The assay contains functionally-verified oligonucleotide primers and unlabeled probes necessary for PCR and subsequent allele identification by DNA melting. For Research Use Only. Not for use in diagnostic procedures.

Catalog number

 

40317 

 

Gene symbol

 

CYP3A5 

Alias

 

Cytochrome P450, Family 3, Subfamily A, Polypeptide 5, Cytochrome P450, Subfamily IIIA (Niphedipine Oxidase), Polypeptide 5, Cytochrome P450 HLp2, Cytochrome P450-PCN3 

Nucleotide change

 

c.1035dupT 

Protein change

 

Allele information

 

*7 

Application area

 

Pharmacogenetics

Variant type

 

Indel

Cytogenetic location

 

7q21.1

SNP type

 

Duplication 

 

NCBI 1000 Genomes Browser

 

rs41303343

dbSNP ID

 

ClinVar

 

Online Mendelian Inheritance in Man (OMIM)

 

 

PharmGKB

 

Drug interaction

 

 

Product specifications

The CYP3A5 c.1035dupT Novallele Genotyping Assay is compatible with any thermocycler capable of high-resolution melting (HRM). Each assay comprises a single tube containing the primers and probes optimized to detect the target. Each tube contains 600 µl, which is enough for 166 reactions. The assay is stable for 6 months at 2–8ºC.

Genotyping analysis

The CYP3A5 c.1035dupT Novallele Genotyping Assay identifies a duplication of c.1035dupT. The graphs display the homozygous wild type in black, the heterozygous variant in blue, and the homozygous variant in red. The data were generated using the companion Novallele Control Set.

Resources

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