AssayDetailPortlet

CYP2C9 c.1003C>T (UP) Novallele Genotyping Assay

Gene symbol

CYP2C9 

Nucleotide change

c.1003C>T 

Protein change

NCBI 1000 Genomes Browser

rs28371685

Assay catalog number

40385 

Control set catalog number(s)

40725

The CYP2C9 c.1003C>T (UP) Novallele Genotyping Assay identifies a single mutation associated with the CYP2C9*11 allele. The CYP2C9 gene provides instructions for making an enzyme involved in protein processing and transport. The CYP2CP enzyme plays a major role in breaking down the drug warfarin and assists in metabolizing drugs such as ibuprofen. Variations of the CYP2C9 gene that decrease the activity of the CYP2C9 enzyme can result warfarin sensitivity. The altered enzyme slows the breakdown, or metabolism, of warfarin, allowing the drug to remain active in the body for a longer period of time.

Variant information

The CYP2C9 c.1003C>T (UP) Novallele Genotyping Assay is used to amplify and identify specific genetic polymorphisms or mutations. This assay provides scientists with a simple way to quickly genotype this locus from isolated human genomic DNA. The assay contains functionally-verified oligonucleotide primers and unlabeled probes necessary for PCR and subsequent allele identification by DNA melting. For Research Use Only. Not for use in diagnostic procedures.

Catalog number

 

40385 

 

Gene symbol

 

CYP2C9 

Alias

 

Cytochrome P450 Family 2 Subfamily C Member 9, Cytochrome P450, Family 2, Subfamily C, Polypeptide 9, Cholesterol 25-Hydroxylase, Cytochrome P450 PB-1, Cytochrome P-450MP, CYP2C10, CYPIIC9, Cytochrome P450, Subfamily IIC (Mephenytoin 4-Hydroxylase), Polypeptide 9, Cytochrome P-450 S-Mephenytoin 4-Hydroxylase, Flavoprotein-Linked Monooxygenase, (R)-Limonene 6-Monooxygenase, (S)-Limonene 6-Monooxygenase, (S)-Limonene 7-Monooxygenase, S-Mephenytoin 4-Hydroxylase, Microsomal Monooxygenase, Xenobiotic Monooxygenase, Cytochrome P450 MP-4, Cytochrome P450 MP-8, EC 1.14.13.80, EC 1.14.13.48, EC 1.14.13.49, EC 1.14.99.38, EC 1.14.14.1, EC 1.14.13.-, P450IIC9, CYP2C, CPC9 

Nucleotide change

 

c.1003C>T 

Protein change

 

Allele information

 

*11 allele 

Application area

 

Pharmacogenetics

Cardiovascular

Variant type

 

SNP

Cytogenetic location

 

SNP type

 

Missense 

 

NCBI 1000 Genomes Browser

 

rs28371685

dbSNP ID

 

ClinVar

 

Online Mendelian Inheritance in Man (OMIM)

 

 

PharmGKB

 

Drug interaction

 

phenytoin, warfarin 

Product specifications

The CYP2C9 c.1003C>T (UP) Novallele Genotyping Assay is compatible with any thermocycler capable of high-resolution melting (HRM). Each assay comprises a single tube containing the primers and probes optimized to detect the target. Each tube contains 600 µl, which is enough for 166 reactions. The assay is stable for 6 months at 2–8ºC.

Genotyping analysis

The CYP2C9 c.1003C>T (UP) Novallele Genotyping Assay identifies a SNP of c.1003C>T. The graph displays the homozygous wild type in black, the heterozygous variant in blue, and the homozygous variant in red. The data were generated using the companion Novallele Control Set.

Resources

Related products

  • ©   Canon BioMedical, Inc. All Rights Reserved. Reproduction in whole or part without permission is prohibited.