AssayDetailPortlet

CYP2A6 c.497T>A Novallele Genotyping Assay

Gene symbol

CYP2A6 

Nucleotide change

c.497T>A 

Protein change

p.Leu160His

NCBI 1000 Genomes Browser

rs1801272

Assay catalog number

40387 

Control set catalog number(s)

40727

The CYP2A6 c.497T>A Novallele Genotyping Assay detects a single nucleotide change of the CYP2A6 gene. The CYP2A6gene codes for an enzyme that is known to hydroxylate coumarin and also metabolizes nicotine, aflatoxin B1, nitrosamines, and some pharmaceuticals. Individuals with certain allelic variants have a poor metabolizer phenotype, meaning they do not efficiently metabolize coumarin or nicotine.

Variant information

The CYP2A6 c.497T>A Novallele Genotyping Assay is used to amplify and identify specific genetic polymorphisms or mutations. This assay provides scientists with a simple way to quickly genotype this locus from isolated human genomic DNA. The assay contains functionally-verified oligonucleotide primers and unlabeled probes necessary for PCR and subsequent allele identification by DNA melting. For Research Use Only. Not for use in diagnostic procedures.

Catalog number

 

40387 

 

Gene symbol

 

CYP2A6 

Alias

 

Cytochrome P450 Family 2 Subfamily A Member 6, Cytochrome P450, Subfamily IIA (Phenobarbital-Inducible), Polypeptide 6, Cytochrome P450, Family 2, Subfamily A, Polypeptide 6, 1,4-Cineole 2-Exo-Monooxygenase, Coumarin 7-Hydroxylase, Cytochrome P450 IIA3, Cytochrome P450(I), CYPIIA6, CYP2A3, Flavoprotein-Linked Monooxygenase, Xenobiotic Monooxygenase, EC 1.14.14.1, EC 1.14.13.-, P450C2A, P450PB, CYP2A, CPA6 

Nucleotide change

 

c.497T>A 

Protein change

 

p.Leu160His

Allele information

 

*2 allele 

Application area

 

Pharmacogenetics

Variant type

 

SNP

Cytogenetic location

 

19q13.2

SNP type

 

Missense 

 

NCBI 1000 Genomes Browser

 

rs1801272

dbSNP ID

 

ClinVar

 

Online Mendelian Inheritance in Man (OMIM)

 

 

PharmGKB

 

Drug interaction

 

coumarin 

Product specifications

The CYP2A6 c.497T>A Novallele Genotyping Assay is compatible with any thermocycler capable of high-resolution melting (HRM). Each assay comprises a single tube containing the primers and probes optimized to detect the target. Each tube contains 600 µl, which is enough for 166 reactions. The assay is stable for 6 months at 2–8ºC.

Genotyping analysis

The CYP2A6 c.497T>A Novallele Genotyping Assay identifies a SNP of c.497T>A. The graph displays the homozygous wild type in black, the heterozygous variant in blue, and the homozygous variant in red. The data were generated using the companion Novallele Control Set.

Resources

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