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Since PCR was introduced, DNA replication has incrementally gotten easier and faster. Now, a revolution has happened. Next-generation PCR from Molecular Biology Systems (MBS) heats and cools instantly — "ramp rates" no longer exist. Using one thermocycler, you can run more than ten plates per hour. Go from melting to annealing in less than one tenth of a second. Complete PCR in two to ten minutes and process more samples per day by incorporating NEXTGENPCR into your workflow.


Copy Number Variation

Rapid PCR with high-resolution melting (HRM) analysis enables scientists to quickly determine copy number variations in their genes of interest. We developed a functionally verified chemistry that supports faster ramping rates and shortened cycling times as compared to typical PCR conditions. Using just an HRM-enabled thermocycler, you can run a single reaction to determine copy number­—no sample replicates required. 


Copy Number Assays

Copy Number Mastermix


With the Novallele genotyping assays, you can detect genetic variations, such as single-nucleotide polymorphisms (SNPs) and both small and large indels, of the human genome using any thermocycler capable of HRM. The Novallele genotyping assays contain functionally verified oligonucleotide primers and probes necessary for rapid PCR and the subsequent allele identification by HRM analysis.

Genotyping Assays

Genotyping Controls

Genotyping Reagents

HRM Analysis

Reliably analyze your HRM data using our free, web-based software. The Novallele HRM Analyzer features an intuitive, easy-to-use graphical interface with comprehensive algorithms capable of analyzing HRM data from various thermocyclers.

Novallele HRM Analyzer

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