HRM SNP Assays - A Swift and Simple Option

Save time and money with Novallele assays

High-resolution melting (HRM) analysis provides a fast answer for known genetic variations, such assingle-nucleotide polymorphisms (SNPs) and indels, while also being cost effective and simple. The combination of inexpensive DNA dyes, improved primer design, intelligent automation, and sophisticated analysis software has made detecting mutations easier. With most labs already having HRM-enabled thermocyclers, adding HRM analysis to your laboratory does not require significant investment or training.

Copy Number Analysis - Simplified

Accurate and reproducible copy number calls

The Novallele copy number assays generate accurate and reproducible copy number calls using HRM-enabled thermocyclers without requiring multiple technical replicates for each sample. The assays are fast — copy number calls are determined in under an hour.

Genotype Your Toughest Targets

Rapid reactions deliver quick results

The Novallele genotyping assays detect genetic variations, SNPs and both small and large indels, of the human genome using an HRM-capable thermocycler. Partnering with academic centers and commercial organizations, Canon BioMedical developed a genotyping library linked to the diverse fields of human biology.

Application Data

Do You Need to Genotype a Single Sample in an Hour?

Start melting your sample today

Genotyping using HRM analysis is a simple and fast method that detects variants in nucleic acid samples. A majority of PCR genotyping methods require a minimum number of samples to achieve reliable genotyping results. Unlike most other genotyping techniques, HRM analysis can quickly genotype as little as one sample at a time.

Evaluating Critical Performance Factors Between Three Different Instruments for HRM Genotyping Assays

Start melting your sample today

PCR followed by HRM is a fast, reliable, and inexpensive method to perform DNA genotyping. Multiple manufacturers have added HRM capabilities to their thermocyclers. The HRM-based Novallele genotyping assays for research were developed using the Bio-Rad CFX384 Touch. Our data demonstrate that carefully designed and optimized HRM assays are easily transferred between different thermocyclers, expanding the utility of these assays and HRM analysis as a method.

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